IRTA’s Plant in Vitro Culture laboratory has focused on the application of plant tissue culture techniques to assist and hasten private or public plant breeding programs. Among these techniques, micropropagation, embryo rescue, generation of doubled haploid lines and tetraploids, genetically modified plants, and generation of plant cell cultures, are offered.
Services and Applications
Micropropagation: propagation of individuals, rootstocks or varieties, selected from field plots or out of conventional breeding programs. Applied mainly to fruit trees (Pyrus spp, Prunus spp., Malus spp., Juglans regia, Pistacia vera, Actinidia deliciosa, Jatropha curcas), and ornamentals.
Embryo rescue: recovery of viable plants from immature embryos derived from crosses among early ripening peach and nectarine varieties. Applicable to distant interspecific crosses of ornamentals, vegetables, or fruit trees.
Generation of doubled haploid lines and poliploids: generation, via androgenesis or parthenogenesis, of doubled haploid lines (DHLs), useful to produce homozygous lines, which are parents of commercial hybrid varieties. This service is offered for several vegetable crops, and could be applied to ornamentals such as carnation. Production of tetraploid lines is offered for vegetable crops and ornamentals.
Genetic transformation: Agrobacterium-mediated genetic transformation is available for certain genotypes of rice, grape, melon, pepper, tomato and tobacco. The protocols are offered to confirm the functionality of cloned genes.
Cell cultures: generation, maintenance, and production of plant cell cultures from different parts of various plant species.